Abstract Background CRISPRi screening has become a powerful approach for functional genomic research. However. the off-target effects resulting from the mismatch tolerance between sgRNAs and their intended targets is a primary concern in CRISPRi applications. Results We introduce Guide Library Designer (GLiDe). https://www.bekindtopets.com/flash-offer-FreeTress-Equal-Headband-Full-Cap-Wig-HARMONI-amazing-save/