Repeated Cycles of amplification bring about degradation of your probe, causing increased fluorescence. TaqMan detection is, consequently, a lot more precise than dye‐based detection, since an increase in fluorescence happens only upon binding on the probe to a certain sequence and subsequent de‐quenching, though no sign is made by amplification https://www.facebook.com/NewTechFind/posts/pfbid0KSgSCxKeWVcVHuBsw8R21p5Rbehtn77wq3EKWFh2eqeB43kuVWCWLM5NXrmahaBJl